Get On-demand Access to Perkin Elmer Opera Phenix Microscope

The Opera Phenix by Perkin Elmer is a fully-integrated hardware and software system for high throughput automated confocal/epifluorescent/digital phase cell-based or tissue or organism multiwell imaging. The HTSF’s system can be environmentally controlled by temperature and 5% CO2 within the microscope. Delivery of plates from either a Liconic incubator or “hotel” stacks by a robotic plate handler can be set up for day or overnight imaging. The Screening Facility staff trains researchers on the Harmony cellular image analysis software and Columbus for high content analysis, although researchers are free to use whatever analysis pipeline that they prefer using the .tif images. Objective selections include 2x, 4x, 10x, 20x Air, 20xWater, 40x Air, high NA 40x Water, and 60x Water, and an array of emission filter capabilities including DAPI, CFP, GFP, FITC, YFP, TRITC, Cy3, TxRed, and Cy5. This microscopy system is also set up for FRET in any number of combinations. Imaging immunofluorescence slides or plate wells with randomly positioned areas to image is made easy by the use of the Opera Phenix Preciscan routine that quickly images at low magnification, automatically recognized features of interest, and then re-images only those areas of interest at higher magnification and resolution.