Full Service Cryo-Ultramicrotomy projects taken on a case by case basis and as time allows within the lab.
Ultramicrotomy with:
The EML has Leica ultramicrotomes (UC6 and UC7), also equipped with cryo-ultramicrotomy mount, for sectioning samples. Material/polymer (as in https://doi.org/10.1016/j.geoen.2023.212607) and/or biological specimen cryo-sectioning available.
50nm – 100nm (or more) sections can be collected onto a variety of grid types as needed (slot or mesh), using Diatom diamond knives.
For projects that need correlation back to whole tissues, we employ toluidine blue staining of “thick” sections collected onto glass slides, light microscopy images are taken for reference.