The Agilent BioTek Lionheart FX cellular imaging system is a fully-integrated hardware and software system for medium throughput automated cell-based still and video imaging–wide-field, brightfield, phase, digital phase, high-contrast phase for wound healing cell counting, color (like H&E staining) for fixed/live cells. The flexibility of this systems makes it useful for use with fluidic devices, slides, and other setups.
The CTAF’s system has environmental control of temperature, CO2 and O2 (hypoxia) and is equipped with 1.25x, 4x, 10x, 20x, and 40x oil immersion magnification, laser autofocus, and filter sets for DAPI, GFP, FITC, TRITC, TxRed, and Cy5. The CTAF Lionheart FX uses Gen 5 Prime cellular image analysis software for high content analysis.
This powerful system complements the IXM in that it can be used with more bulky sample holders and rigs, with hypoxia experiments, easy slide imaging and montaging, and when different fields need to be imaged in each well (using “beacons”).
