The Bruker Prairie Swept Field Confocal (SFC) system brings both high spatial resolution and high temporal resolution together into a single compact confocal scanner. Users can control the acquisition time and laser exposure with software control, as well as the frequency and duration of each “sweep” of the scanner. Highest spatial resolution is obtained by using the pinhole size best suited to a given objective lens. Z-and T-series are easily set up with scan speeds in excess of 30 frames per second (fps). Confocal laser lines currently in place are 405, 488, 561, and 643 nm.
A Warner Instruments/Harvard Apparatus perfusion and heated plate stage is also available, delivering 5% CO2 to the medium that will be perfused across the live sample in either an open configuration (QE-1) for direct access to the Prairie Technologies 2-Photon/SFC upright microscope system, or enclosed between glass coverslips (Quick Change). The Six Channel Perfusion Valve Control Systems (VC-6) setup is for medium to low flow, and allows easy change over between different media compositions during imaging.
