Great for a non-wet lab who needs to do accurate Protein/Peptide quantitation. The Direct Detect system provides more accurate results without the pitfalls of colorimetric assays. By measuring amide bonds in protein chains, the IR spectrometry system accurately quantitates an intrinsic component of every protein without relying on amino acid composition, dye binding properties or reduction-oxidation (redox) potential. You can evaluate major components of complex mixtures separable from the Amide I & II region, like lipids and carbohydrates—making accurate quantitation of lysates and membrane preps compared to Bradford or BCA assays.
● More compatible with detergents, reducing agents and other buffer components that interfere with traditional protein quantitation assays, providing more information from your sample
● Preserves precious samples—requires only 2 μL per analysis
● Also quantifies long aliphatic chains in lipids and detergents, so good for purification evaluations
