The Bio-Rad CFX Connect is for single-color FAM, EvaGreen, and SYBR Green I Assays, the fast-scan option reads single-channel fluorescence in all 96 wells in just 3 seconds. A separate channel with an LED filter–photodiode combination is dedicated to FRET singleplex experiments.
The newer CFX Opus (acquired in 2024) is a 5 color instrument for a wider range of probes and multiplexing.
The CTAF qPCR Systems features six independently controlled thermal electric units, providing even, precisely controlled temperatures at all times during the run, including ramping. The programmable thermal gradient feature allows a temperature gradient of up to 24°C across the reaction block at any step in a protocol. Uses low-profile strip tubes or plates.
The optical system, with three filtered LEDs and three filtered photodiodes, collects data from all wells, detecting up to two targets per well. For single-color FAM, EvaGreen, and SYBR Green I Assays, the fast-scan option reads single-channel fluorescence in all 96 wells in just 3 seconds. A separate channel with an LED filter–photodiode combination is dedicated to FRET singleplex experiments.
