Get On-demand Access to Manual Epifluorescence/Color Microscopy - Zeiss AxioObserver A1

NOTE: Access to UC Berkeley facilities and services requires prior university approval which takes as little as 24 hours! Select "Request Access" button to begin the process and contact support@secondlab.com with any questions.

Once approved, each user may be required to complete instrument-specific training. See listing descriptions for more details.

All external, non-UCB customers are required to comply with all UC visitor policies and lab rules. Please read the following Access Guidelines taken from the Schedule A of our CTAF specific Agreement. For those requiring regular access is important to understand that we are a small facility with limited storage and access so discussion and a tour with the Lab Manager before booking the first time is highly recommended. Please contact mwest@berkeley.edu.

Berkeley Research Infrastructure Commons CTAF Access Agreement Shared User Facility

University agrees to give Company and the Company Users named below Access to QB3 CTAF as follows:

Company has access to the instrumentation below, following approval from the CTAF Director:

Cell Culture Room

Scepter Cell Counter 3.0 by Millipore

Mammalian Cell Culture Room and accessories

Biospherix Hypoxia Chambers

Eppendorf TransferMan

Liquid Nitrogen Dewar for cell line storage

Microscopy

Automated Epifluorescence Microscope and Analysis Workstation, ImageXpress Micro XLS by Molecular Devices

Agilent Lionheart Multi-functional Epifluorescence Microscope for Cells and Tissue Slides Bruker Swept-Field Confocal/2P Microscope

Zeiss Epifluorescent Microscope

EVOS xL Core Bright Field and Color Microscope

Flow Cytometry/Mass Photometry

ThermoFisher Attune NXT 4 laser

Refeyn 2MP Mass Photometer

Protein Instrumentation

Molecular Devices Multilabel SpectraMax Plate Reader

BioRad Bioplex MagPix (Luminex XMap technology)

DNA Instrumentation

BioRad CFX Connect and Opus qPCR (Realtime PCR)

BioRad Thermal Cycler

BioRad Electroporator Gene Pulser Xcell for bacteria, yeast, mammalian cell Tissue Preparation

Miltenyi GentleMACS tissue dissociator, DNA and RNA extraction

Cryostats, microtomes, and Histology Staining Area (chemical hood area)

Miscellaneous

“Hot Bench” – hourly use Chemical Fume Hood 

Company is given access to the CTAF to utilize the above listed instruments for research, development, proto-typing, and analysis.

Entrance into the CTAF facility will be during building access hour from 8 am – 6 pm unless special arrangements are made with the CTAF manager, and reservations based on availability will be made using iLab scheduling web interface. Use is typically 3-4 times weekly for 1-2 hr/day, more often if using the Cell Culture Room.

Parties agree that no more than 3 staff will be in any one of the rooms (fewer if Covid19 precautions are in effect) at a given time to prevent overcrowding.

Company may be given access to CTAF facilities to utilize the above listed cell culture and analysis equipment for both fixed and live samples to further their research and development efforts. It is Company's responsibility to submit the BUAs and send a copy of it and the approval letter to the CTAF before research with live cells or recombinant DNA commences.

Disposal and pickup of all biomedical waste will be the Company’s responsibility.

CTAF staff will only provide training and expertise with respect to instrumentation scheduling and performance, and there will be no assistance provided by CTAF staff with respect to the researcher’s scientific decision making. All pertinent CTAF Standard Operation Procedures (SOPs) will be read and followed and consumables and reagents will be stored and disposed of appropriately. Data will be deleted after adequate time for analysis and/or backup has elapsed.

If the Company wants CTAF staff to provide services, Company will be required to submit a separate RIC Service Agreement (in addition to this RIC Access Agreement), that will detail the specific services requested.

All external, non-UCB customers are required to comply with all UC visitor policies and lab rules. Please read the following Lab Rules taken from the Schedule A of our CTAF Agreement. This is important for understanding that we are a small facility with limited storage and access so discussion with the Lab Manager before booking first time is important. Please contact mwest@berkeley.edu.

Berkeley Research Infrastructure Commons CTAF Access Agreement Shared User Facility

University agrees to give Company and the Company Users named below Access to QB3 CTAF as follows:

Company has access to the instrumentation below, following approval from the CTAF Director:

Cell Culture Room

Scepter Cell Counter 3.0 by Millipore

Mammalian Cell Culture Room and accessories

Biospherix Hypoxia Chambers

Eppendorf TransferMan

Liquid Nitrogen Dewar for cell line storage

Microscopy

Automated Epifluorescence Microscope and Analysis Workstation, ImageXpress Micro XLS by Molecular Devices

Agilent Lionheart Multi-functional Epifluorescence Microscope for Cells and Tissue Slides Bruker Swept-Field Confocal/2P Microscope

Zeiss Epifluorescent Microscope

EVOS xL Core Bright Field and Color Microscope

Flow Cytometry/Mass Photometry

ThermoFisher Attune NXT 4 laser

Refeyn 2MP Mass Photometer

Protein Instrumentation

Molecular Devices Multilabel SpectraMax Plate Reader

BioRad Bioplex MagPix (Luminex XMap technology)

DNA Instrumentation

BioRad CFX Connect and Opus qPCR (Realtime PCR)

BioRad Thermal Cycler

BioRad Electroporator Gene Pulser Xcell for bacteria, yeast, mammalian cell Tissue Preparation

Miltenyi GentleMACS tissue dissociator, DNA and RNA extraction

Cryostats, microtomes, and Histology Staining Area (chemical hood area)

Miscellaneous

“Hot Bench” – hourly use Chemical Fume Hood 

Company is given access to the CTAF to utilize the above listed instruments for research, development, proto-typing, and analysis.

Entrance into the CTAF facility will be during building access hour from 8 am – 6 pm unless special arrangements are made with the CTAF manager, and reservations based on availability will be made using iLab scheduling web interface. Use is typically 3-4 times weekly for 1-2 hr/day, more often if using the Cell Culture Room.

Parties agree that no more than 3 staff will be in any one of the rooms (fewer if Covid19 precautions are in effect) at a given time to prevent overcrowding.

Company may be given access to CTAF facilities to utilize the above listed cell culture and analysis equipment for both fixed and live samples to further their research and development efforts. It is Company's responsibility to submit the BUAs and send a copy of it and the approval letter to the CTAF before research with live cells or recombinant DNA commences.

Disposal and pickup of all biomedical waste will be the Company’s responsibility.

CTAF staff will only provide training and expertise with respect to instrumentation scheduling and performance, and there will be no assistance provided by CTAF staff with respect to the researcher’s scientific decision making. All pertinent CTAF Standard Operation Procedures (SOPs) will be read and followed and consumables and reagents will be stored and disposed of appropriately. Data will be deleted after adequate time for analysis and/or backup has elapsed.

If the Company wants CTAF staff to provide services, Company will be required to submit a separate RIC Service Agreement (in addition to this RIC Access Agreement), that will detail the specific services requested.