Insect cell service for protein production (High5 or SF9)

NOTE: Access to UC Berkeley facilities and services requires university approval which takes as little as fast as 24 hours. First time customers on Second Lab will be prompted to complete approval requirements during checkout. We encourage you to book your 15 minute onboarding with the Second Lab team through the following link: Click here to schedule your onboarding (15 mins)!

During checkout, you will be presented with a form for the respective service you're requesting. Additional requirements may be applicable if your service requires physical access to University of California facilities.

Q: When is the latest I should place my order for: An insect cell suspension line? 

An insect cell suspension line? 

A: HIGH5 (split M, W, F): by 9am on the split day you would like it to be ready. For orders greater than the equivalent of 1x1L @ 1e6, please place your order by 9am the split day prior to your pick up date (e.g. A 2L HIGH5 order with a density of 1e6/ml for pick up on a Wednesday should be placed by 9am the Monday before. A Monday pick up for the same order should be placed by 9am the Friday before). 

SF9 (split M, W, F): by 9am on the split day you would like it to be ready. For orders greater than the equivalent of 2x1L @ 1e6, please place your order by 9am the splitting day prior to your pick up date (Assuming no larger orders are in front of your order).

EXPI SF9 (split M, Th): by 9am on the split day prior to your pick up date. For orders greater than the equivalent of 100ml @ 2e6, please place your order by 9am at least TWO splitting days prior to your pick up date. For orders greater than the equivalent of 1.5L @ 1e6, please place your order at least FOUR splitting days (~2 weeks) before your pick up date. 

*for pick up dates that are not on a split day (e.g. Tues), you should place orders as if they are to be picked up on the split day prior (e.g. A HIGH5 order <1L @1e6/ml for pick up Tuesday should be placed Monday by 9am. A HIGH5 order >1L @ 1e6/ml for pick up Tuesday should be placed Friday by 9am.)

A mammalian suspension line? 

293 GNTI (split M,Th): by 9am the splitting day prior to your pick up date

EXPI F, EXPI GNTI: (split M, Th): by 9am the splitting day prior to your pick up date

*Please note larger orders (293 GNTI: >100ml @ 1e6, EXPI F: >125ml @ 1e6) will take additional time. These orders should be placed at least a week in advance. But we suggest at least 2 weeks especially for 293 GNTI orders larger than the equivalent of 2L @ 1e6/ml)

Q: Do I need to do anything after I place my order?

A: If you plan to use recycled flasks for your suspension lines, please bring them at least two splitting days in advance. We do not keep black well plates. If you request them for an order, you must provide your own a day in advance. 

Q: When will my order be ready?

A: You can expect your order to be ready on your desired pick up date. For same day orders, pick up is after 2pm. If there is a delay, we will inform you ahead of time. For mycoplasma and testing and STR validation, please allow up to a week before results. 

Q: Will you contact me when my order is ready? 

A: No. Once you place your order, expect that it will be ready by 2pm on the day you requested. The only exceptions are suspension lines that require us to thaw the same day. Those will be ready by 4:30pm at the latest. We will only contact you if there is a reason for delay or need to clarify a request.